Fig. 3

IκBα-mediated metabolic rewiring and apoptotic responses through p65-NF-κB. a Upper panel: Western blot analysis of extracts from A549 cells infected with shCTR, shIΚBD2 and shA10 p65 immunostained for IκBα, p65 and vinculin as a loading control; Lower pane: quantification of p65/IκBα protein levels. Data are shown as mean ± sem (n ≥ 3 independent experiments). P-values are from Student’s t-test. *P < 0.05. b A549 cells infected described in (a), were analyzed for ETC complexes activity, lactate production (c), lipoperoxidation (d) and cholesterol esters production (e). Data are shown as mean ± sem (n ≥ 3 independent experiments). P-values are from Student’s t-test. *P < 0.05; **P < 0.01. f Percentage of apoptosis in A549 cells previously described in (a) subjected for 48 h to cisplatin treatment, assessed by Annexin V. Data are shown as mean ± sem (n ≥ 3 independent experiments). P-values are from Student’s t-test. *P < 0.05; ***P < 0.001