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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Glioma stem cells invasive phenotype at optimal stiffness is driven by MGAT5 dependent mechanosensing

Fig. 2

Optimal migration of GSCs on 166 kPa NFS. Migration was estimated by subtracting the area of the initial NS to the total area occupied by the NS and cells having migrated away from it within 5 days. a, b F-Actin was stained with the Phalloïdin (Green) and the nucleus stained with the Hoechst 33342 (Blue). a Left panel: in migration medium (Scale bar: 200 μm). Right panel: in proliferation medium (Scale bar: 200 μm). b A view of cells migrating on the 166 kPa NFS at higher magnification. Collective migration were considerate for each aggregates composed at least by tens cells tightly associated (18). Arrows point at a few examples of collective migration (Scale bar: 100 μm). c 3000 dissociated GSCs were seeded on NFSs of different stiffnesses and proliferation was estimated with the MTT assay after 5 days of culture. No significant differences between conditions could be discerned (n = 3). d Collective migration quantification. e Migration areas in migration medium as a function of NFS stiffness showing appreciable migration only at 166 kPa (n = 3 with at least 6 GSCs NS per condition). f Migration areas in proliferation medium as a function of NF stiffness showing appreciable migration only at 166 kPa (n = 3 with at least 4 GSCs NS per condition). Values of the figure correspond to the mean ± SEM and statistical significance was determined using one-way ANOVA, (* p < 0,05; ** p < 0,01; *** p < 0,005; **** p < 0,001)

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