Fig. 3

Optimal stiffness increases EMT and FA maturation in GSCs. a GSCs were cultured on 2D and NFSs of different stiffnesses. Labelling: vinculin green, actin red (phalloidin) and DNA blue (Hoechst 33342). b Length, c Width and d aspect ratio of GSCs grown fibres of different stiffnesses were determined. Measurements were taken regarding to nucleus location. (n = 3 with at least 172 cells by condition) Values of the figure correspond to the mean ± SEM and statistical significance was determined using one-way ANOVA (* p < 0,05; ** p < 0,01; *** p < 0,005; **** p < 0,001). e Western Blot membranes showing EMT and focal adhesion protein expression in GSCs grown on NFSs with different stiffnesses. (n = 3 to n = 7 for each protein tested) Associated quantifications in Supp. Figure 2