Fig. 4

KO of MGAT5 decreases GSC migration at 166 kPa. a Western blot of MGAT5 expression in WT and 4 MGAT5 KO clones (n = 3). b LC-MS/MS data for total bi- (N2H2), tri- (N3H3) and tetra- (N4H4) antennary N-glycans expressed as a ratio of MGAT5 KO / WT reveals the loss of N4H4 and addition of polylactosamine >N4H4; (* p < 0.05). Our analysis does not distinguish lactosamine isomer, which are therefore represented as minor structures in structures with >N3H3 units. The N-glycans with masses >N3H3 in KO cells, are most likely N2H2 and N3H3 with polylactosamine extensions with identical masses to N4H4 (n = 3). c Proliferation of WT and MGAT KO GSCs does not differ (n = 5). d Compared to WT GSC, NS formation is inhibited in MGAT5 KO GSCs. e Migration of WT and MGAT KO GSCs on fibres of various stiffnesses. F-Actin was stained with Phalloïdin (Green) and the nucleus stain with Hoechst 33342 (Blue). f Quantification of migration area in differentiation medium comparing WT and MGAT5 KO GSCs showing a significant reduction of migration of KOs at 166 kPa (n = 3 with at least 6 GSCs NS per condition). Values of the figure correspond to the mean ± SEM and statistical significance was determined using one-way ANOVA (* p < 0,05; ** p < 0,01; *** p < 0,005; **** p < 0,001)