Fig. 5

MGAT5 KO modifies the expression of EMT and adhesome proteins. a MGAT5 KO GSCs were cultured on 2D and NFSs of different stiffnesses. Labelling: vinculin green, actin red (phalloidin) and DNA blue (Hoechst 33342). b Length, c Width and d aspect ratio of MGAT5 KO GSCs grown fibres of different stiffnesses were determined and compared to WT. Asterisks indicate significant differences between KO and WT (n = 3 with at least 86 cells by condition). e Western Blot of EMT and adhesome protein expression by MGAT5 KO GSCs (n = 3 to n = 4 for each protein tested). Associated quantifications in Supp. Figure 4. Values of the figure correspond to the mean ± SEM and statistical significance was determined using one-way ANOVA (* p < 0,05; ** p < 0,01; *** p < 0,005; **** p < 0,001)