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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Hypoxia-dependent expression of MAP17 coordinates the Warburg effect to tumor growth in hepatocellular carcinoma

Fig. 2

MAP17 promotes the Warburg effect in HCC cells. a The knockdown efficiency of 5 independent shRNAs against MAP17 in SMCC-7721 and HCC-LM3 cells was verified by western blotting analysis. b-e Effect of MAP17 knockdown on the glucose uptake (b, n = 3), lactate release (c, n = 3), extracellular acidification rate (d, n = 3), and expression of glycolytic genes (HK2, ENO1, and LDHA; e, n = 3) in SMCC-7721 and HCC-LM3 cells. f The overexpression efficiency of MAP17 and its PDZ binding domain mutant MAP17-PDZm in MHCC-97H cells was confirmed by western blotting analysis. g-j Effect of MAP17 overexpression on the glucose uptake (g, n = 3), lactate release (h, n = 3), extracellular acidification rate (i, n = 3), and expression of glycolytic genes (j, n = 3) in MHCC-97H cells. *P < 0.05 and **P < 0.01

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