Fig. 4

Knocking down OXCT1-AS1 markedly suppresses the proliferation of GBM cells in vivo. a OXCT1-AS1 expression levels in GBM cell lines compared with those in the human brain astrocyte cell line NHA. b Three shRNAs targeting OXCT1-AS1 and the empty vector were transfected into A172 and LN229 cells. The inhibitory efficiency was measured by qRT-PCR. c, f After transfecting sh-OXCT1-AS1 and vector, CCK-8 cell proliferation assays were performed at 0, 24, 48, and 72 h. d, e Colony formation assays performed using A172 and LN229 cells transfected with sh-OXCT1-AS1 and the empty vector. g, h Fluorescence microscopy of Ki67 expression after silencing OXCT1-AS1 or transfection with the empty vector. The scale bar represents 100 μm. The bars represent SD (*p < 0.05, **p < 0.01, ***p < 0.001)