Fig. 6

Knocking down OXCT1-AS1 suppresses CDC25A, reduces CDK2 phosphorylation and induces cell cycle arrest. a-d Flow cytometry assays were performed to analyse the cell cycle progression of the A172 and LN229 cell lines transfected with sh-OXCT1-AS1 or the empty vector. The cells in the different cell cycle phases were assessed and are shown in the bar chart. e Expression of cell cycle-related proteins, such as CDC25A, CDK2, CCNA1 and CCNE1, in OXCT1-AS1 knockdown cells. f Using total CDK2 as the internal control, the expression levels of phosphorylated CDK2 were calculated. The bars represent SD (*p < 0.05, **p < 0.01, ***p < 0.001)