Fig. 3

Down-regulation of ALG3 improves radiosensitivity. a Cell viability of SUM159PT and MDA-MB-231 irradiated with 0, 2, 4, 6, 8 and 10 Gy X-rays and cultured for 72 h were detected by CCK-8 assays. Data were analyzed by two-way ANOVA with Bonferroni’s post test. Each bar represents the mean ± SD of three independent experiments. b Caspase-3 and Caspase-9 activity in MDA-MB-231 and SUM159PT cells. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. Each bar represents the mean ± SD of three independent experiments. c Apoptosis ratio of MDA-MB-231 and SUM159PT cells after treated with 2 Gy X-rays and cultured for 72 h was detected by flow cytometry. Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test. Each bar represents the mean ± SD of three independent experiments. d The clonogenic survival fraction curve was fitted using a multitarget single-hit statistical model by colony assay. Data were analyzed by two-way ANOVA with Bonferroni’s post test. e Representative fluorescence graph of tumor-bearing mice with different ALG3 expression, which were treated with 2 Gy X ray. f Tumor growth rate was significantly decreased in ALG3-knocked out cells after radiation treatment. Data were analyzed by two-way ANOVA with Bonferroni’s post test. g Representative pictures of tumors from ALG3-sg + IR and control + IR mice were as shown. h The tumor weight of the ALG3-sg group after radiation treatment and the tumor weight of the control group after radiation treatment were calculated and shown. Data were analyzed by Student’s t-test. i The fluorescence intensity of OCT4 in ALG3-sg cells relative to that in control cells were measured. Data were analyzed by Student’s t-test. *P < 0.05, **P < 0.01, *** P < 0.001