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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: SLC6A8-mediated intracellular creatine accumulation enhances hypoxic breast cancer cell survival via ameliorating oxidative stress

Fig. 4

SLC6A8 mediates intracellular creatine accumulation in hypoxic TNBC cells. a Intracellular creatine concentrations in hypoxic and normoxic MDA-MB-231 and BT549 cells. b, c Lentivirus-mediated shRNA against Slc6a8 (shSlc6a8) or scrambled control (shNC) was stably expressed in TNBC cells, the knockdown efficiencies of SLC6A8 were verified by qRT-PCR (b) and western blot (c). d Intracellular creatine concentrations were determined in shNC and shSlc6a8 TNBC cells cultured in media containing 10% FBS under normoxia or hypoxia for 24 h. e Determination of creatine concentrations in the media derived from normoxic or hypoxic MDA-MB-231 and BT549 cells transfected with shNC or shSlc6a8. f The Slc6a8 parental or silenced TNBC cells that were cultured in media containing 1% FBS were additionally administered creatine (5 mM) or PBS under normoxia or hypoxia condition for 24 h, and intracellular creatine concentrations were determined. g Determination of intracellular creatine concentrations in tumor tissues of TNBC patients based on high and low expression of Slc6a8 mRNA level as stratified by relative median value of Slc6a8 mRNA level in TNBC tissues. Data were presented as mean ± SD (**P < 0.01, ***P < 0.001)

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