Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: SLC6A8-mediated intracellular creatine accumulation enhances hypoxic breast cancer cell survival via ameliorating oxidative stress

Fig. 5

SLC6A8-mediated creatine accumulation promotes TNBC cell survival under hypoxia. shNC and shSlc6a8 TNBC cells were treated with or without 5 mM creatine (Cr) and exposed to normoxia or hypoxia for 24 h, then cell viability and apoptosis were assessed. a Cell viability was determined by CCK8 assay. b, c Cell apoptotic rate was determined using Annexin V/PI double staining. d The levels of Ki-67, Bcl-2, Bax and cleaved Caspase-3 (cleaved CASP3) were detected by western blot. Data were presented as mean ± SD (*P < 0.05, **P < 0.01)

Back to article page