Fig. 2

Sec62 promotes the stemness of CRC cells. a-b Spheres derived from DLD1 or HT29 cells treated as indicated (left). The summary of the number and size of spheres (right) (n = 5). c-d Spheres derived from DLD1 or HT29 cells stably expressing Sec62 or vector cells (left). The summary of the number and size of spheres (right) (n = 5). e Western blot analysis was performed to determine the expression of levels of indicated CSC markers in Sec62 knockdown and control cells. f CD133⁺/CD44⁺ cells were analyzed by flow cytometry in DLD1 or HT29 cells stably transcfected with Sec62 shRNA or control shRNA. g DLD1 Sec62-shRNA or DLD1 Ctrl shRNA cells were diluted and subcutaneously implanted into nude mice. Tumorigenic cell frequency was analyzed with a limiting dilution assay