Fig. 4

Sec62 stabilizes β-catenin through repressing its degradation. a Whole cell lysates extracted from the Sec62-depleted cells or control cells were subjected to Western blot using the indicated antibodies. b Total RNAs were extracted from Sec62-depleted and control cells and RT-qPCR were performed for evaluating the mRNA levels of β-catenin. c The Sec62-depleted and control cells were treated with MG132 and cell lystaes were subjected to Western blot performed with the indicated antibodies. d The Sec62-depleted and control shRNA cells were cultured in Wnt3a (0.2 μg/mL) contained medium for 6 h. Cell lysates were subjected to Western blot performed with the indicated antibodies. e-f DLD1 cells were transfected with Flag-Sec62 and its deletion mutants as indicated. Cells were harvested and cell lysates were subjected to Western blot using the indicated antibodies. g The Sec62-depleted and control cells were treated with MG132. The ubiquitination level of β-catenin was analyzed as indicated. h The Sec62-depleted and control RKO cells were treated with MG132. The ubiquitination level of β-catenin was analyzed as indicated. i-j DLD1 (i) or RKO (j) cells were transfected as indicated and treated with MG132. The ubiquitination level of β-catenin was analyzed as indicated