Fig. 5

Sec62 disrupts the β-catenin destruction complex. a Phosphorylation level of β-catenin in Sec62-depleted CRC cells was analyzed by immunoblotting (left). The ratio of phosphorylated β-catenin/total β-catenin (P/T) is summarized (right) (n = 3). b Phosphorylation level of β-catenin in Sec62-depleted RKO or HEK293 cells was detected by immunoblotting after Wnt3a treatment (n = 3). c DLD1 cells were transfected with indicated plasmids and phosphorylation level of β-catenin was analyzed by western blot (n = 3). d HT29 cells were transfected with indicated plasmids and co-IP was performed with the cell lysates using anti-β-catenin antibody. Western blot was performed on the precipitates using the indicated antibodies. e HEK293 cells were transfected with indicated plasmids and cell lysates were subjected to co-IP using anti-Flag antibody. f DLD1 cells were transfected with indicated plasmids and cell lysates were subjected to co-IP using anti-β-catenin antibody. g The Sec62-depleted and control cells were harvested and cell lysates were subjected to co-IP using anti-β-catenin antibody. h DLD1 cells transfected with indicated plasmids and cell lysates were subjected to co-IP using anti-APC antibody. i RKO cells were transfected with Flag-Sec62 or Flag vetor and cultured with Wnt3a medium. The cell lysates were subjected to co-IP using anti-APC antibody