Fig. 6

Sec62 promotes CRC progression by activating β-catenin signaling. a DLD1 cells were transfected with indicated vectors. Cell fraction was performed and the nuclear β-catenin was evaluated by Western blot. b DLD1 cells were treated as indicated. The promoter occupancy of LGR5 or MYC by β-catenin was analyzed by ChIP assay. c The Sec62 depleted and control cells were transfected with Sec62 plasmid. RT-qPCR was performed to analyze the mRNA levels of the indicated genes. d The Sec62-depleted and control cells were treated with Wnt3a. Total RNAs were extracted and RT-qPCR was performed as indicated. e The Sec62-depleted and control cells were treated with iCRT14 (50 μM for 24 h). RT-qPCR was performed to analyze the mRNA levels of the indicated genes. f Tumor formation in nude mice injected with Sec62-overexpressed, Sec62 DA-overexpressed DLD-1 or control cells. g The tumor growth curves of each group of mice were summarized (n = 5). h Total RNAs were extracted from the xenografts obtained in (F). RT-qPCR was performed for evaluating the indicated genes (n = 5). i-j DLD1 cells were infected with indicated lentivirus and sphere formation assays were performed in sphere-formation medium (i). The number of spheres derived from indicated cells were summarized (j) (n = 6). k Colony number quantification of the DLD1 cells after treatment with 5-Fu or oxaliplatin (n = 6)