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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: ROC1 promotes the malignant progression of bladder cancer by regulating p-IκBα/NF-κB signaling

Fig. 3

ROC1 overexpression activated NF-κB signaling and promoted the expression of target genes related to metastasis. a ROC1 knockdown increased p-IκBα protein expression (left). Conversely, p-IκBα expression decreased following ROC1 overexpression (right). *P < 0.05, **P < 0.01, two-tailed Student’s t-test; Tukey’s post-hoc test was performed following one-way ANOVA. b, c WB and qRT-PCR analysis showed elevated transcript and protein levels of uPAR, ICMA1, VCAM1, and MMP9 in ROC1-overexpressing T24 and 5637 cells. Conversely, ROC1 knockdown showed opposite effects. β-actin served as internal control. The data are represented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, two-tailed Student’s t-test; Tukey’s post-hoc test was performed following one-way ANOVA. d ROC1 knockdown reduced the nuclear location of p65 (red), whereas ROC1 overexpression increased the nuclear location of p65 (red). Nuclei were counterstained with DAPI (blue). The left picture shows T24 cells and the right picture shows 5637 cells. Cells were counted in six randomly visual fields. Scale bar, 100 μm. The results are expressed as the mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001, two-tailed Student’s t-test. e. Cells with differing ROC1-expression levels were harvested at different time points after LPS treatment (0, 30, 60, or 120 min), and the nuclear translocation of p65 and other NF-κB-related markers were determined by WB. GAPDH and H3 were used as the cytoplasmic and nuclear markers, respectively

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