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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: ROC1 promotes the malignant progression of bladder cancer by regulating p-IκBα/NF-κB signaling

Fig. 5

NF-κB inhibition and IκBα knockdown eliminated the difference in invasiveness of BCa cells induced by ROC1 overexpression. a, c Transwell-invasion assays for ROC1-overexpressing T24 cells treated with BAY 11–7082 (or DMSO) and siIκBα (or siNC, a negative-control siRNA). Representative images of invaded cells are shown, and the results are summarized for each experimental condition. At least three independent experiments were performed, and images of the invaded cells from representative experiments are shown. Scale bar, 100 μm. b The histogram shows the number of invaded cells/field after treatment with the indicated chemicals and siRNAs. All results are expressed as the mean ± SD of three independent experiments. ***P < 0.001, two-tailed Student’s t-test. d WB was performed to detect cytoplasmic p-IκBα and nuclear p65 expression after the cells were treated with the indicated chemicals and siRNAs. H3 and GAPDH were used as nuclear and cytoplasmic markers, respectively

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