Fig. 2

Timeless promotes cell proliferation and invasion in vitro. (a) The expression levels of Timeless in seven CRC cell lines were analyzed by western blotting. (b) Timeless expression was detected by Western blotting in HCT116 and RKO cells transfected with the indicated siRNAs or pcDNA-Tim. (c) CCK-8 assay and (d) colony-forming growth assays were performed using HCT116 and RKO cells transfected with Timeless siRNAs or pcDNA-Tim. Transwell assays were performed to detect the migration (e) and invasion (f) of Timeless-knockdown and overexpressing cells after 36–48 h. Data represent the mean ± standard deviation from three independent experiments. *P < 0.05, **P < 0.01