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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Activation of the clock gene TIMELESS by H3k27 acetylation promotes colorectal cancer tumorigenesis by binding to Myosin-9

Fig. 5

Timeless activates Wnt/β-catenin signaling. Western blotting was performed to detect total (a) and nuclear β-catenin (b) in Timeless knockdown and overexpressing CRC cells. (c) The laser scanning confocal microscope was performed to detect the subcellular location of β-catenin. Original magnification, × 400 (d) Expression levels of Axin2, CCND1, LEF1 and CTNNB1 were assayed by qRT-PCR in the indicated cells. An immunoprecipitation (IP) assay was performed to detect Flag-tagged β-catenin (e) or (f) Flag-tagged Timeless that interacted with Timeless or β-catenin in HCT116 cells. Error bars indicate the means ± SD. *P < 0.05, **P < 0.01

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