Fig. 2

PMN depletion abolishes RvD1 anti-cancer effects. a Scheme of the in vivo transplantation of C3 cells in the right flank of C57BL/6 mice, treated with vehicle or RvD1 plus anti-IgG or -Ly6G antibodies three times a week. b Representative IF analysis of tumor slides from mice treated with anti-IgG or anti-Ly6G and stained with an anti-elastase antibody to detect tumor-infiltrated PMN. Nuclei were counterstained with DAPI. Tumor edges were marked with dotted lines. Scale bar: 50 μM. c Left and middle: Representative flow cytometric counter plot of single-cell dissociated tumors from mice treated with anti-IgG or anti-Ly6G, as indicated, and identified using the strategy reported in Fig. S6. Right: Percentage of tumor-infiltrated PMN determined by FACS analysis of CD66a⁺ cells. Data are expressed as percentage of CD11b⁺ cells. n = 4–7 mice per group. *, P < 0.05 (two-tailed unpaired t test). d Tumor growth of C3 cells in mice treated with vehicle/RvD1 plus anti-IgG/−Ly6G antibodies. Data are expressed as volume fold change as compared to the initial tumor volume at the start of treatments (T1). n = 5–6 mice per group. * P < 0.05 vehicle+IgG vs RvD1 + IgG at day 11 (two-way ANOVA and Tukey’s multiple comparison test); ** P < 0.01 RvD1 + IgG vs RvD1 + Ly6G at day 11 (two-way ANOVA and Tukey’s multiple comparison test). d Kaplan-Meier curve survival analysis reporting the time for tumors to reach a volume of 1500 mm³ (humanized endpoint). n = 6–7 mice tumors per group. ***, P < 0.001 RvD1 + IgG vs RvD1 + Ly6G (Log-rank Mantel-Cox test)