Fig. 7
Cotreatment with NAC and PLZ decreased liver metastasis in the xenografted organoid model with PSC co-implantation. a The spleens of nude mice were implanted with KPC mouse-derived organoids (1 × 105) and mouse PSCs (1 × 105). After 1 week, mice were intravenously injected with vehicle (100 μl PBS + 0.1 μl DMSO, n = 5), NAC (500 mg/kg, n = 5), PLZ (4 mg/kg, n = 5), or their combination (n = 5) once every 2 days for 21 days. On day 28, we sacrificed the mice and evaluated their hepatic metastasis. b Representative photographs showed the number of disseminated visible nodules in the liver, and liver volume was decreased after combined treatment with NAC and PLZ. c-d The metastasis nodules (c) and the tumor weight and volume (d) in mice liver were evaluated. e H&E staining, Masson’s staining trichrome and Immunohistochemical staining of α-SMA and CK19 were performed. *P < 0.05; **P < 0.01; ***P < 0.001; n.s, no significance