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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Inhibition of retinoic acid receptor α phosphorylation represses the progression of triple-negative breast cancer via transactivating miR-3074-5p to target DHRS3

Fig. 2

Phosphorylation-defective RARα-mediated suppression of TNBC cell growth in vitro is independent of ligand-activation. a Western blotting analysis of RARα (p-Ser77) and total RARα expression levels after overexpression with RARα hypo-phosphorylated mutant (RARαS77A), wild-type RARα, and empty vector. b Cell viability after overexpression of RARαS77A, RARα, and empty vector in the presence or absence of different RARα agonists were determined by MTT analysis. RARαS77A versus Control, *** p < 0.001. c TNBC cells overexpressing RARαS77A, RARα, and empty vector were further cultured with AM580 (0, 1 μM) for 14 days. The colony formation was observed by crystal violet staining. d The relative number of colonies in panel C was quantified. e qRT-PCR analysis of transcriptional expression of direct RA-target genes. * p < 0.05, ** p < 0.01, *** p < 0.001

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