Fig. 1

circ_0072083 expression is increased in TMZ-resistant glioma tissues and cells. a The proportion of MGMT methylation was analyzed in TMZ-resistant or sensitive tissues. b circ_0072083 level was detected via qRT-PCR in TMZ-resistant (n = 36) or sensitive tissues (n = 33). c and d Cell viability and IC50 of TMZ were measured via CCK-8 in cells after treatment of various doses of TMZ. e circ_0072083 abundance was examined in TMZ-resistant or sensitive cells. f The information of circ_0072083 was analyzed via CircView. g and h circ_0072083 and ZFR mRNA levels were detected via qRT-PCR after treatment of Actinomycin D for different time points or RNase R. i circ_0072083 abundance was examined via qRT-PCR in cytoplasm and nucleus with U6 and 18 s rRNA as references. j and k The si-circ_0072083#1, si-circ_0072083#2 and si-circ_0072083#3 were constructed by targeting the splice junction, and their knockdown efficacy was detected via qRT-PCR. ^*P < 0.05