Fig. 3

circ_0072083 knockdown decreases NANOG expression by regulating ALKBH5-mediated demethylation. a ALKBH5 and NANOG mRNA levels were detected via qRT-PCR in TMZ-resistant (n = 36) or sensitive tissues (n = 33). b ALKBH5 and NANOG levels were measured by qRT-PCR in TMZ-resistant and sensitive cells. c and d NANOG mRNA m6A enrichment level was analyzed in TMZ-resistant or sensitive tissues and cells. e and f ALKBH5 and NANOG protein levels were examined by western blot in TMZ-resistant or sensitive tissues and cells. g and h ALKBH5 and NANOG levels were examined via qRT-PCR and western blot in resistant cells transfected with sh-ALKBH5 or sh-NC. i NANOG mRNA enrichment level was detected in resistant cells after RIP assay using anti-ALKBH5 or anti-IgG. j NANOG mRNA m6A enrichment level was examined in resistant cells transfected with sh-ALKBH5 or sh-NC. k NANOG mRNA level was measured via qRT-PCR in cells with transfection of sh-ALKBH5 or sh-NC after treatment of Actinomycin D for different time points. l and m ALKBH5 and NANOG levels were detected via qRT-PCR and western blot in cells transfected with sh-circ_0072083 or sh-NC. n NANOG mRNA m6A enrichment level was detected in cells transfected with sh-circ_0072083 or sh-NC. o The schematic diagram of circ_0072083-regulated NANOG through ALKBH5-mediated demethylation. ^*P < 0.05