Fig. 5

The release of exosome is associated with Warburg effect in resistant glioma cells. a The secretion of exosome was confirmed via TEM. Scale bar: 200 nm. b The concentration and size of exosome were analyzed. c CD63, CD81, TSG101 and GM130 protein levels were detected via western blot in exosome from cells. d Exosomal circ_0072083 level was detected via qRT-PCR in resistant or sensitive cells. e and f Lactate production and glucose uptake levels were measured in resistant or sensitive cells. g and h GLUT1, LDHA and PKM2 levels were examined via qRT-PCR and western blot in resistant or sensitive cells. i and j Exosome concentration and exosomal circ_0072083 level were examined in resistant cells after stimulation of Shikonin, TNF-α or PBS. ^*P < 0.05