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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: KLF5-induced BBOX1-AS1 contributes to cell malignant phenotypes in non-small cell lung cancer via sponging miR-27a-5p to up-regulate MELK and activate FAK signaling pathway

Fig. 3

BBOX1-AS1 induces cell proliferation, migration, invasion and EMT in NSCLC. a After transfection with si-NC or siRNAs aganist BBOX1-AS1 (si-BBOX1-AS1#1 or si-BBOX1-AS1#2), NSCLC cells were subjected to qRT-PCR for detecting BBOX1-AS1 expression level. b and c CCK-8 and colony formation assays were carried out to assess cell proliferation potential upon BBOX1-AS1 knockdown. d Wound healing assays were used to evaluate the impact of BBOX1-AS1 depletion on cell migration. e Transwell assays were applied to detect cell invasion ability in the present of siRNAs targeting BBOX1-AS1. f Western blot assays were utilized to the measure the expression of EMT markers (E-cadherin, N-cadherin and vimentin) in NSCLC cells with low BBOX1-AS1 expression. **P < 0.01, ***P < 0.001

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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