Fig. 4

FAPα+ and CD26+ CAF subpopulations dominate the TME at all times. Top 5 most abundant Boolean CAF subpopulations (subsets) in a 4T1 and b 4T07 tumours at a given time point (within the CAF+ population). The CAF population becomes less heterogeneous over time, and FAPα (green) and CD26 (red) expressing subsets dominate the TME. Mean % and SEM from three independent repeats combined. One hundred twenty-eight tumours in total. 4T1 D7 n = 21, 4T1 D14 n = 24, 4T1 D21 n = 24, 4T07 D7 n = 24, 4T07 n = 21, 4T07 D21 n = 14. See supplementary materials for complete abundance ranking of all 63 CAF subsets