Fig. 2

The tumor immune cell composition differs between PD and CD patients. a Representative images of 7-color mIHC staining of metastatic UM samples derived from PD and CD patients. First panel (above): CD3 in magenta, CD20 in white, CD68 in yellow, neutrophil elastase in red, CD56 in green, melanoma mix in cyan, nuclei in blue. Second panel (below): CD4 in yellow, CD8 in magenta, Granzyme B in green, FoxP3 in red, CD163 in white, melanoma mix in cyan, nuclei in blue. Original magnification 20X. b Immune cell quantitation from the two mIHC panels plotted as the percentage of cells in PD and CD patients, calculated in the intra-tumoral and stromal regions. The mean values and the standard deviations are represented. c CD8+/CD4+ cell ratio calculated in the intra-tumoral and stromal compartments in PD and CD patients. d Intra-tumoral/Peri-tumoral (I/P) ratio of CD8 + Granzyme+ T lymphocytes in PD and CD patients. e Intra-tumoral CD8+ T cells density (number of cells/mm²) in PD and CD patients. f Mean distance (μm) between each UM cell (stained positive for the melanoma cocktail of antibodies) and the nearest CD3+ T lymphocyte in PD and CD patients. g Percentage of UM cells within a radius of 25 μm from CD8+ T lymphocytes in PD and CD patients. Floating box extends from 25th to 75th percentiles, line through the box indicates median, and bars extend from the smallest to largest values. Non-parametric Mann-Whitney statistical analysis was performed across the two groups, and significantly different data are represented by *(p < 0.05)