Fig. 3

The tumor immune cell composition differs according to the site of UM metastases. a Immune cell quantitation from the two mIHC panels plotted as percentage of cells in LM and EM, calculated in the intra-tumoral and stromal regions. The mean values and the standard deviations are represented. b-c Intra-tumoral cell densities (number of cells/mm²) of CD163+ M2-polarized macrophages (b) and CD8+ T lymphocytes (c) in LM and EM. d Percentage of intra-tumoral activated CD8 + Granzyme B+ T lymphocytes among intra-tumoral total CD8+ cells in LM and EM. e Mean distance (μm) between each UM cell (stained positive for the melanoma cocktail of antibodies) and the nearest CD8+ T lymphocyte in LM and EM. f-g Percentage of UM cells within a radius of 20 μm from CD8+ T lymphocytes (f) and from CD163+ TAMs (g) in LM and EM. h Percentage of CD8+ T lymphocytes within a radius of 20 μm from CD163+ TAMs in LM and EM. Floating box extends from 25th to 75th percentiles, line through the box indicates median, and bars extend from the smallest to largest values. Non-parametric Mann-Whitney statistical analysis was performed across the two groups, and significantly different data are represented by *(p < 0.05), **(p < 0.01)