Table 1 List of primary antibodies used in mIHC staining
From: The immune cell landscape of metastatic uveal melanoma correlates with overall survival
Antigen | Panel | Clone | Vendor |
|---|---|---|---|
Neutrophil Elastase (NE) | 1st | NP57 | Dako |
CD56 | 1st | NP57 | Dako |
CD3 | 1st | F.7.2.38 | Dako |
CD20 | 1st | L26 | Dako |
CD68 | 1st | KP1 | Dako |
CD8 | 2nd | C8/144B | Dako |
CD4 | 2nd | 4B12 | ThermoFisher |
Granzyme B | 2nd | 11F1 | Leica Biosystems |
FoxP3 | 2nd | D2W8E | Cell Signalling |
CD163 | 2nd | 10D6 | Leica Biosystems |
Anti-melanoma mix (HMB-45 + Mart-1 + Tyrosinase + SOX-10) | 1st and 2nd | HMD45 + M2-7C10 + M2-9E3 + T311 + EP268 | Abcam and Cell Marque |
Spectral DAPI | 1st and 2nd | Â | Akoya Biosciences |