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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: ZBTB28 induces autophagy by regulation of FIP200 and Bcl-XL facilitating cervical cancer cell apoptosis

Fig. 2

Tumor suppressive functions of ZBTB28 in cervical cancer cells. a RT-PCR and western blot analysis confirmed the exogenous expression of ZBTB28 in CaSki and HeLa cells, with β-actin as a control. b Ectopic ZBTB28 expression impacts cervical cancer cell growth were analyzed by the CCK8 kit. c Re-expression of ZBTB28 suppressed colony formation in CaSki and HeLa cells. d Morphological changes of cervical cancer cells that stable transfected with pcDNA-3.1 or pcDNA-ZBTB28 by using phase contrast microscopy. e EMT marker were detected by RT-PCR and western blot assay. f, g Pictures were taken at 48 h after seeding, the relative ratio of migration and invasion cells per field was shown. h Representative spheroid-forming cells images were taken at 14 days after seeding. i Overexpression of ZBTB28 in cervical cancer cells downregulated the mRNA expression level of stemness-related genes. All statistical data were shown as mean ± SEM. Scale bar: 200 μm; *p < 0. 05, **p < 0. 01, ***p < 0. 001

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