Fig. 5

Blocking ZBTB28-induced autophagy attenuated apoptosis in cervical cancer cells. a The data were shown as representative TEM images of cervical cancer cells which transfected with pcDNA-3.1 or pcDNA-ZBTB28. Arrows pointed the characteristic of autophagosomes. (20,000x, Scale bar: 1 μm). b Stable transfected cells were transient transfected with EGFP or EGFP-LC3B. Photographs were taken under a confocal microscopy. c, d Stable transfected cells were treated with CQ (20 μM) or 3MA (10 mM) for 24 h. p62, LC3 were detected by western blot. β-actin was used as negative control. e, f Stable transfected cells were treated with CQ (20 μM) or 3MA (10 mM) for 24 h. Casp8, Casp3, and PARP were detected by western blot. β-actin was used as negative control. g Forty eight after transfection with siBECN1 or control siRNA, the expression of BECN1, p62, LC3, Cleaved-casp8, Casp3, Cleaved-casp3 and Cleaved-PARP were evaluated by western blot. β-actin was used as negative control. h Stable transfected CaSki and HeLa cells were treated with CQ (1 μM) or re-transfected with siBECN1 and control siRNA. Seventy two hours after treatment, cell viability was assessed by CCK8 assay. *p < 0. 05, **p < 0. 01, ***p < 0. 001