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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: ZBTB28 induces autophagy by regulation of FIP200 and Bcl-XL facilitating cervical cancer cell apoptosis

Fig. 7

ZBTB28 upregulated FIP200 to induce autophagy and apoptosis. a qRT-PCR results of FIP200 mRNA expression in stable transfected cervical cancer cells. b Structure of wild-type and mutant FIP200 reporter plasmid. c % Input of FIP200 DNA by HA-tag antibody were tested by ChIP-PCR. Then products of ChIP-PCR were used for electrophoresis. d The effect of ZBTB28 on FIP200 promoter activity was detected by dual luciferase reporter system. e CaSki and HeLa cells were transfected with pcDNA3.1 or ZBTB28, then tested promoter luciferase activity of mutant FIP200 in live cells. f Forty eight hours after transfection with siFIP200 or control siRNA, the expression of FIP200, p62, LC3, Cleaved-casp8, Casp3, Cleaved-casp3 and Cleaved-PARP were evaluated by western blot. β-actin was used as negative control. g Stable transfected CaSki and HeLa cells were re-transfected with siFIP200 or control siRNA. Seventy two hours after transfection, cell viability was assessed by CCK8 assay. *p < 0. 05, **p < 0. 01, ***p < 0. 001

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