Fig. 3

Identification of PUS7 downstream targets in CRC cells. a Heatmap of the PUS7 downstream targets assessed by RNA-seq analysis. Red refers to the upregulation of the corresponding genes, while blue refers to the downregulation. b Bubble plot of KEGG enrichment terms based on RNA-seq results. The alteration in colour from red to blue illustrates a decrease in the P-value, while the size of the circles indicates the number of genes enriched in KEGG terms. c Heatmap of the PUS7 downstream targets assessed by proteome profiling analysis. Red refers to the upregulation of the corresponding genes, while green refers to the downregulation. d Bubble plot of KEGG enrichment terms based on proteome profiling results. The alteration in colour from red to blue illustrates a decrease in the P-value, while the circle size designates the number of genes enriched in KEGG terms. e Volcano plot showing the proteins that changed significantly between PUS7 knockdown DLD1 and control cells. f Levels of expression of LASP1 between tumour and non-malignant tissues of COAD and READ by GEPIA analysis. g Correlation analysis of PUS7 and LASP1 mRNA expression levels in the GEPIA database. h PUS7 and LASP1 expression in the specified cell lines. i PUS7 and LASP1 expression in 7 pairs of CRC tumour and non-malignant control tissues. j Images illustrating IHC staining for LASP1 in human CRC tissues and neighbouring non-malignant tissues, and corresponding statistical analysis. k Images illustrating IHC staining for PUS7 and LASP1 expression in CRC tissues and corresponding correlation analysis. l, m qPCR l and WB m analyses of the LASP1 expression in the specified stable cell lines. n Co-localization detection of PUS7 and LASP1 performed by immunofluorescence (IF) analysis