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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: HSP90-dependent PUS7 overexpression facilitates the metastasis of colorectal cancer cells by regulating LASP1 abundance

Fig. 6

HSP90 binds to PUS7 and increases the PUS7 protein levels in CRC cells. a Bubble plot of GO enrichment terms. The alteration in colour from red to blue illustrates a decrease in the P-value, while the size of the circles indicates the number of genes enriched in GO terms. b DLD1 and HCT-8 were transfected with or without LV-PUS7. Co-IP was performed to determine the relationship between PUS7 and HSP90. c WB analysis of HSP90 in the specified cell lines. d WB analysis of HSP90, PUS7, and LASP1 in the specified cell lines. e DLD1 and HCT-8 cells were treated with NMS-E973 (1 μM) and WB was performed with anti-PUS7 antibodies at the specified time points. f DLD1 and HCT-8 cells were exposed to different levels of NMS-E973 as specified and WB was performed with anti-PUS7 antibodies after 24 h of treatment. g Selected IHC staining images for HSP90 in CRC matched tissues and relative statistical analysis h Selected IHC staining images for HSP90, PUS7, and LASP1 in CRC paired tissues and correlation analysis. i Co-localisation of HSP90 and PUS7 performed by IF analysis. All the data are shown as the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001

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