Fig. 7

HSP90 regulates the protein stability of PUS7 in CRC cells. a CHX-chase assay showing the degradation of PUS7 in DLD1 and HCT-8 cells treated with NMS-E973 (1 μM) or control DMSO. b CHX-chase assay showing the degradation of PUS7 in DLD1 and HCT-8 cells transfected with LV-HSP90 or control LV-NC lentivirus. c, d CQ (20 μM) was used to inhibit lysosomal degradation c and MG-132 (100 nM) was employed to repress proteasomal degradation d in DLD1 cells. MG-132 treatment reversed the downregulation of the PUS7 protein levels triggered by HSP90 suppression. All the data are shown as the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001