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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma

Fig. 4

High expression of DEC2 was necessary for CoCl2- induced dormancy. a CCK-8 analysis of SACC-83 cells treated with 100, 300, 500 and 700 μM CoCl2. b and c The expression of Ki-67 and cell cycle of CoCl2 treated cells were determined by immunofluorescence and flow cytometry. d Cell growth analysis of SACC-83 cells treated with 500 μM CoCl2 for 7 days (from day 4 to day 10) and then recovered in normal media. e Cell growth analysis of SACC-83 cells treated with 0.1% O2 for 7 days (from day 4 to day 10) and then recovered into normoxia environment. f The expression of DEC2, HIF1α, P27, P53, NR2F1, CDK4, P38, ERK, Slug, Snail, Twist, Zeb, E-cadherin and N-cadherin were determined by QPCR in SACC-83/LM cells treated by CoCl2 and the control group. g The protein levels of DEC2, HIF1α and Slug in CoCl2 treatment and control SACC-83 cells. h DEC2 expression at different concentrations of CoCl2 in SACC-83. i and j Proliferation capacity and cell cycle were determined by CCK-8 assay (i) and flow cytometry (j). k Migration and invasion abilities of CoCl2 treatment and CoCl2 + siDEC2 SACC-83 cells were detected by Transwell and scratch assays, respectively. l The mRNA levels of Slug and HIF-1α in CoCl2 or CoCl2 + siDEC2 treatment SACC-83 cells. Error bars represent the mean ± SD of triplicate experiments (*P < 0 .05, **P < 0.01, ***P < 0 .001)

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