Fig. 3

Lnc-CTSLP8 promoted autophagy in ovarian cancer cells. a GSEA analysis of SKOV3-CTSLP8-OE and SKOV3-CTSLP8-NC (control vector). The signature was defined by genes with significant expression changes. b Transmission electron microscopy (TEM) was used to observe autophagosomes (AU), autolysosomes (AL), and mitochondria (MT) in different cell lines. c Immunoblotting of autophagy-related genes in different cell lines. d Quantification of immunoblotting analysis revealed the relative SQSTM1-p62 expression and LC3-II/ LC3-I ratio in different cell lines (Student t-test). e Immunofluorescence assay of mRFP-GFP-LC3 in different cell lines