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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: The lnc-CTSLP8 upregulates CTSL1 as a competitive endogenous RNA and promotes ovarian cancer metastasis

Fig. 6

Lnc-CTSLP8 upregulated CTSL1 expression in ovarian cancer by acting as a ceRNA. a qRT-PCR of CTSL1 expression in benign ovarian cyst tissues (used as control), ovarian cancer tissues, and matched peritoneal metastasis tissues. b The correlation between lnc-CTSLP8 and CTSL1 expression levels in different tissues. c GEO data analysis of the correlation between CTSL1 expression and lnc-CTSLP8 expression in ovarian cancer. d Prediction of shared miRNAs and miRNA binding sites within lnc-CTSLP8 and CTSL1 3’UTR sequences. e Biotinylated wild-type/mutant miR-199a-5p was transfected into SKOV3 cells overexpressing CTSLP8. After streptavidin capture, CTSLP8 and CTSL1 expression were detected via qRT-PCR. f RIP experiments were performed using an anti-AGO2 antibody and SKOV3 cells. g Luciferase reporter assay using the 3’UTR of CTSL1 and CTSLP8 to analyze repression by the indicated miRNA mimics. Non-specific control miRNA (miR-NC) served as a negative control. h qRT-PCR of CTSL1 expression in different cell lines

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