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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Interferon- and STING-independent induction of type I interferon stimulated genes during fractionated irradiation

Fig. 1

Induction of a type I IFN response in cancer cells peaks after 2 weeks of fractionated irradiation in vitro. Fractionated irradiation induces a type I IFN response in cancer cells, which peaks after 2 weeks and coincides with a convergence in clonogenic survival to a steady state. a Scheme of fractionated irradiation applied to human cancer cells in vitro. b Clonogenic survival analyses show a log-linear decline in survival during the first 2 weeks of treatment after which a steady-state survival is reached up to 6 weeks of treatment. Adapted from Van den Berg et al. [11]. c Heat map showing the 30 most downregulated and upregulated genes after 2 weeks of treatment vs. untreated as determined by RNA deep-sequencing of HT29 cells (n = 3). d The mRNA expression induction of a panel of 10 IFN-stimulated genes (ISGs) after 2 weeks of treatment was confirmed by qPCR (n = 3). Geometric mean + SD is shown. * p-value ≤0.05 vs. no radiotherapy (RTx). e Time course analysis of ISG mRNA expression induction shows a peak starting around 2 weeks of treatment (n = 3). Geometric mean + SD is shown. * p-value ≤0.05 vs 0 × 2 Gy

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