Fig. 3

Inhibition of CXCL12/CXCR4 signaling blocked the effects of miR-455-5p inhibitor on MTC cell proliferation, migration, and invasion. a Relative CXCL12 mRNA and protein levels in MTC cells transfected shNC or shCXCL12. b & c Representative images of colonies formed in MTC cells transfected miR-455-5p mimics or shCXCL12 or treated with CXCR4 antagonist AMD3100. d Flow cytometry analysis of the number of transfected cells in G1, S, and G2 phases. e Flow cytometry analysis of cell apoptosis in MTC cells transfected miR-455-5p mimics or shCXCL12 or treated with CXCR4 antagonist AMD3100. f & g Transwell assay to analyze the migration and invasion abilities of transfected cells. h & i EMT-related protein levels in MTC cells transfected miR-455-5p mimics or shCXCL12 or treated with CXCR4 antagonist AMD3100. * P < 0.05; ** P < 0.01; *** P < 0.001