Fig. 3

MYLK4 promotes osteosarcoma cell proliferation. a Osteosarcoma tissues from mice with tumor xenografts inoculated with MYLK4-knockdown 143B cells. The weight of osteosarcoma samples was shown in the down panel. Statistically significant differences (t-test), **P < 0.01. b Western blotting were performed to detect the shRNA interference efficiency in vivo. c The expression of Ki67 was detected by IHC in orthotopic OS implanted-mice tumor tissues formed by MYLK4-knockdown 143B cells and control cells. The results of Ki67 positive (%) were shown in the right panel. The bars indicate the mean ± s.d. Statistically significant differences (t-test), **P < 0.01, Scale bars = 100 μm. d The effect of MYLK4 overexpressing on OS cell proliferation was assessed by the CCK8 assay. The bars indicate the mean ± s.d. Statistically significant differences (t-test), **P < 0.01. e The effect of MYLK4 overexpression on colony formation in OS cells. Representative images of colony formation were shown in the left panel. The numbers of cells in the indicated groups are shown in the histogram. The bars indicate the mean ± s.d. Statistically significant differences (t-test), **P < 0.01. f Cell proliferation was measured by CCK8 assay with/without MYLK4 knockdown in OS cells. The bars indicate the mean ± s.d. Statistically significant differences (t-test), **P < 0.01. g The effect of MYLK4 knockdown on colony formation in OS cells. Representative images of colony formation were shown in the left panel. The numbers of cells in the indicated groups were shown in the histogram. The bars indicate the mean ± s.d. Statistically significant differences (t-test), **P < 0.01