Fig. 4From: MYLK4 promotes tumor progression through the activation of epidermal growth factor receptor signaling in osteosarcomaMYLK4 interacts with EGFR. a Silver staining of whole cell lysates (WCL) and immunoprecipitates (IP) of FLAG-MYLK4 143B cells. The indicated bands were sequenced by mass spectrometry (MS) analysis. b Secondary peptide structure of EGFR acquired by MS. c 143B cells were transfected with Flag-tagged MYLK4 construct and the interaction between exogenous MYLK4 and EGFR was detected by IP and immunoblotting (IB). d Interaction between endogenous MYLK4 and EGFR by IP analyses in 143B cells. e HEK-293 T cells were cotransfected with Flag-tagged MYLK4 and HIS-tagged EGFR constructs, and the interaction between exogenous MYLK4 and EGFR was detected by IP and IBBack to article page