Fig. 5

MYLK4 promotes EGFR phosphorylation and the downstream signaling pathway. a The expression of p-EGFR, p-AKT and p-ERK was detected by western blotting in MYLK4-overexpressing cells. b The expression of p-EGFR, p-AKT and p-ERK was detected by western blotting in MYLK4-knockdown cells. c The expressions of p-EGFR, p-AKT and p-ERK were detected by western blotting with/without EGF stimulation for 15 min. d The expression of p-EGFR was detected by western blotting with EGF (50 ng/ml) stimulation for different time period respectively in MYLK4-knockdown and the control cells. e The expressions of MYLK4 and P-EGFR (Y1068) expression in human OS tissues were detected by IHC assay. Scale bars = 200 μm. f The score of MYLK4 and P-EGFR (Y1068)-positive staining were quantified and the Spearman correlation was performed