Fig. 4
From: Targeting SERT promotes tryptophan metabolism: mechanisms and implications in colon cancer treatment
Serotonin activates mTORC1 through serotonylation. a, b SW480 and HCT116 cells were treated with serotonin for 10 min with or without MDC (TG2 inhibitor) (200 μM) or sertraline (15 μM) pretreated for 30 min. Anti-mTOR was used for IP. Western Blot were probed with anti-serotonin. c WB for mTOR, S6K and SLC1A5 in HCT116 and SW480 cells treated with or without serotonin in the presence or absence of MDC. d WB for mTOR, S6K and SLC1A5 in HCT116 and SW480 cells treated with or without serotonin after transfection with siSERT or negative control