Fig. 4
TRAIL facilitates epithelial-mesenchymal transition (EMT) by regulating the expression of EMT-related proteins. a, b RT-qPCR of EMT-related genes in TRAIL-knockdown KYSE70 and KYSE150 cells. c Western blotting of EMT-related proteins in TRAIL-knockdown KYSE70 or KYSE150 cells and the respective controls. d–f mRNA and protein levels of EMT-related genes in TRAIL-overexpressing EC1 and TE1 cells. g Immunohistochemistry of vimentin and E-cadherin expression in TRAIL-overexpressing EC1 cells and respective controls. NC, negative control. The data are presented as the mean ± SEM. ***P < 0.001, **P < 0.01, *P < 0.05 (unpaired t-test)