Fig. 7

Down-regulating miR-15a-5p promotes M2 macrophage polarization and HCC cell migration, invasion and EMT. a. RT-qPCR detection of miR-15a-5p expression in macrophages after down-regulating miR-15a-5p; b. RT-qPCR detection of M2 macrophages markers (CD206, CD163, CCL17 and CCL18) after down-regulating miR-15a-5p; c. RT-qPCR detection of M1 macrophages markers (TNF-α, IL-6 and TGF-β) after down-regulating miR-15a-5p; d. Transwell assay tested the migration and invasion of SMMC-7721 cells after co-culture with macrophages inhibiting miR-15a-5p; e. Transwell assay tested the migration and invasion of HepG2 cells after co-culture with macrophages inhibiting miR-15a-5p; f. E-cadherin, N-cadherin, vimentin and MMP7 expression in SMMC-7721 and HepG2 cells after co-culture with macrophages inhibiting miR-15a-5p by qPCR; data were expressed as mean ± standard deviation (repetition = 3) and evaluated by One-way AVONA and Tukey’s test. % P < 0.05 compared with the NC-inhibitor group; + P < 0.05 compared with the NC-inhibitor + M group