Fig. 8

DLX6-AS1 mediates miR-15a-5p to target CXCL17 to drive migration, invasion and EMT in HCC. a. RT-qPCR analysis of CXCL17 expression in clinical tissues; b. RT-qPCR and Western blot analysis of CXCL17 expression in cancer cells and normal liver cells; c. RT-qPCR and Western blot analysis of CXCL17 expression in macrophages after down-regulating miR-15a-5p; d. Prediction of the potential binding site of miR-15a-5p and CXCL17 through bioinformatics websites; e. Verification of the targeting relationship between miR-15a-5p and CXCL17 through dual luciferase reporter experiment; data were expressed as mean ± standard deviation (repetition = 3) and evaluated by One-way AVONA and Tukey’s test. $ P < 0.05 compared with HL-7702 cells; & P < 0.05 compared with the NC-inhibitor group