Fig. 9

DLX6-AS1 mediates miR-15a-5p to target CXCL17 to drive migration, invasion and EMT in HCC. a. RT-qPCR and Western blot analysis of CXCL17 expression in macrophages after silencing CXCL17; b. RT-qPCR detection of M2 macrophages markers (CD206, CD163, CCL17 and CCL18) after silencing CXCL17; c. RT-qPCR detection of M1 macrophages markers (TNF-α, IL-6 and TGF-β) after silencing CXCL17;d. Transwell assay tested the migration and invasion of SMMC-7721 cells after co-culture with macrophages inhibiting CXCL17; e. Transwell assay tested the migration and invasion of HepG2 cells after co-culture with macrophages inhibiting CXCL17; f. E-cadherin, N-cadherin, vimentin and MMP7 mRNA expression in SMMC-7721 and HepG2 cells after co-culture with macrophages inhibiting CXCL17; data were expressed as mean ± standard deviation (repetition = 3) and evaluated by One-way AVONA and Tukey’s test; % P < 0.05 compared with the si-NC group; + P < 0.05 compared with the si-NC + M group