Fig. 2

Overexpression of lincSCRG1 dramatically promoted HCC cell proliferation and migration in vitro. Sh-lincSCRG1, sh-NC (in SNU-387 cells), ov-lincSCRG1 and ov-vector (in Hep3B cells) cell lines were established. a Cell viability was examined by MTT assays. b Oncogenic survival was assessed by colony formation assays. c Cell cycle proliferation was evaluated by flow cytometry. d Migration was determined by transwell assays. e Cell cycle-related proteins (CKD4/6 and cyclinD1) and EMT-related proteins (MMP-2/3/9, E-cadherin, N-cadherin and Vimentin) were examined by western blot analysis. In (a-e), */**/***indicates vs. The ov-vector/sh-NC group (*, p < 0.05, **, p < 0.01, ***, p < 0.001)